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Phylogenetic placement of the basidiomycetous yeasts Kondoa malvinella and Rhodosporidium dacryoidum, and the anamorphic yeast Sympodiomycopsis paphiopedili by means of 18S rRNA gene sequence analysis

1994; Elsevier BV; Volume: 35; Issue: 4 Linguagem: Inglês

10.1007/bf02268507

1618-2545

Sung‐Oui Suh, Junta Sugiyama,

Plant Pathogens and Fungal Diseases

The 18S ribosomal RNA gene sequences of the basidiomycetous yeasts Kondoa malvinella and Rhodosporidium dacryoidum, and an anamorphic yeast Sympodiomycopsis paphiopedili were determined. The 18S rRNA gene of R. dacryoidum IAM 13522 (ex type) revealed the presence of an intron-like region with a length of 404 nucleotides, which is presumably assigned to a group I intron. The phylogenetic tree, including 34 published reference sequences, was inferred from 1493 sites which could be unambiguously aligned. The molecular phylogeny, using the ascomycetes as an outgroup, divided the basidiomycetes into three major lineages. The first lineage was composed of the smut fungi (Ustilaginales), represented by Ustilago maydis, U. hordei, and Tilletia caries, including S. paphiopedili. The second lineage included the type species of teliospore-forming yeast genera Leucosporidium, Rhodosporidium, and Sporidiobolus, and the genera Erythrobasidium and Kondoa, both previously included in the Filobasidiaceae. Rhodosporidium dacryoidum showed a close relationship with E. hasegawianum, which was backed by a high bootstrap support. The rust fungi Cronartium ribicola and Peridermium harknessii were also included in this lineage. The last lineage was formed by the filobasidiaceous yeasts, Cystofilobasidium capitatum, Mrakia frigida, Filobasidium floriforme, and Filobasidiella neoformans, and the anamorphic yeasts Bullera alba (the anamorph of Bulleromyces albus) and Trichosporon cutaneum. Members of Tremella and selected hymenomycetous genera were also included in this lineage.