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THE BIOASSAY OF INSULIN IN VITRO BY MANOMETRIC MEASUREMENTS ON SLICES OF MAMMARY GLANDS

1954; Bioscientifica; Volume: 11; Issue: 3 Linguagem: Inglês

10.1677/joe.0.0110269

1479-6805

Judith H. Balmain, C. P. Cox, S. J. Folley, Mary L. McNaught,

Metabolism, Diabetes, and Cancer

SUMMARY The total gas output of slices of lactating rats' mammary glands metabolizing in bicarbonatesaline containing glucose and acetate is increased by the addition of insulin. From this response a straight-line assay has been developed. Using eight rats and twelve respirometers per rat, a six-point assay gave an estimate of relative potency consistent with that obtained by the manufacturers of the preparations used. A value of 0·18 was obtained for the index of precision (λ).