Use of the membrane filter and a filter aid for concentrating and enumerating indicator bacteria and Salmonella from estuarine waters
1976; Elsevier BV; Volume: 10; Issue: 6 Linguagem: Inglês
10.1016/0043-1354(76)90193-7
ISSN1879-2448
AutoresM. W. Presnell, Wallace H Andrews,
Tópico(s)Fecal contamination and water quality
ResumoA membrane filter -Most Probable Number (MF-MPN) procedure for concentrating and enumerating indicator bacteria and Salmonella from estuarine water is described. In Laboratory studies involving estuarine waters the MF-MPN procedure yielded coliform and fecal coliform recoveries comparable to those obtained by the standard multitube MPN procedure. In a study utilizing a pure culture the MF-MPN procedure yielded Salmonella tennessee recoveries comparable to those obtained by a brain heart infusion pour plate agar method involving 10 replicate plates per dilution. In field studies the MF-MPN method consistently isolated coliform and fecal coliform bacteria from waters which were found to yield indeterminately low values for these two groups of bacteria when examined by the standard multitube MPN method. The MF-MPN method detected Salmonella in three polluted estuarine areas and one unpolluted estuarine area. The described MF-MPN method has the following advantages: (1) bacteria can be concentrated from 4 to 5 l. of water within 5–10 min even when turbidities are as high as 120 Jackson Turbidity Units: (2) several bacterial groups can be determined from the same sample: (3) qualitative and quantitative results can be obtained: (4) small volumes of enrichment broth are required.
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