The biuret reaction: Changes in the ultraviolet absorption spectra and its application to the determination of peptide bonds
1962; Elsevier BV; Volume: 3; Issue: 1 Linguagem: Inglês
10.1016/0003-2697(62)90042-8
ISSN1096-0309
Autores Tópico(s)Chemical Synthesis and Analysis
ResumoA modification of the biuret reaction utilizing changes in the ultraviolet spectrum accompanying the reaction has been presented. The method is sensitive to concentrations of as low as 0.01 mg/ml protein, i.e., 10–15 times as sensitive as the visible biuret reaction. A survey of the specificity of the reaction is included.
Referência(s)