Statistical exploration of variation in quantitative two‐dimensional gel electrophoresis data
2004; Wiley; Volume: 4; Issue: 12 Linguagem: Inglês
10.1002/pmic.200300824
ISSN1615-9861
AutoresJ. Gustafsson, Rachel Carmen Ceasar, C. A. Glasbey, Anders Blomberg, Mats Rudemo,
Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoAbstract Two‐dimensional gel electrophoresis is a major technique in global analysis at the protein level. This paper presents an examination of spot volume data from three gel sets with radioactively labeled yeast Saccharomyces cerevisiae proteins. A strong variance versus mean dependence in data was found to be stabilized by applying a shifted logarithmic transformation. However, transformed data showed a remaining substantial variance heterogeneity for different proteins. Furthermore, examination of studentized residuals revealed that transformed data were approximately normally distributed and that there were spatial correlations among the measurement errors in the gel.
Referência(s)