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BIBTEX RIS

Rapid Identification of Sporothrix Species by T3B Fingerprinting

2012; American Society for Microbiology; Volume: 50; Issue: 6 Linguagem: Inglês

10.1128/jcm.00450-12

ISSN

1098-660X

Autores

Manoel Marques Evangelista Oliveira, Paula Sampaio, Rodrigo Almeida‐Paes, Célia Pais, Maria Clara Gutierrez‐Galhardo, Rosely Maria Zancopé‐Oliveira,

Tópico(s)

Yeasts and Rust Fungi Studies

Resumo

ABSTRACT This article describes PCR fingerprinting using the universal primer T3B to distinguish among species of the Sporothrix complex, S. brasiliensis , S. globosa , S. mexicana , and S. schenckii . This methodology generated distinct banding patterns, allowing the correct identification of all 35 clinical isolates at the species level, confirmed by partial calmodulin (CAL) gene sequence analyses. This methodology is simple, reliable, rapid, and cheap, making it an ideal routine identification system for clinical mycology laboratories.

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