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BIBTEX RIS

Microscopia de força atômica aplicada em imunoensaios

2006; Brazilian Chemical Society; Volume: 29; Issue: 1 Linguagem: Inglês

10.1590/s0100-40422006000100024

ISSN

1678-7064

Autores

Antonio Aparecido Pupim Ferreira, Hideko Yamanaka,

Tópico(s)

Analytical Chemistry and Sensors

Resumo

ATOMIC FORCE MICROSCOPY APPLIED TO IMMUNOASSAYS.Affinity reactions have been used for specific detection of their complementary partners and an enormous variety of enzyme-linked immunosorbent assay (ELISA) formats are used in research and in routine serological tests.With the advent of the atomic force microscopy (AFM) technique, the immune reactions have been monitored by these devices.In the present article we focus on applications of AFM to immunoassays.After introducing the basic concepts of AFM, a brief discussion on the monitoring of the interactions between antigens and antibodies through both topographic image and biosensor systems is presented.

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