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Advanced microscale bioreactor system: a representative scale-down model for bench-top bioreactors

2012; Springer Science+Business Media; Volume: 64; Issue: 6 Linguagem: Inglês

10.1007/s10616-012-9446-1

1573-0778

Wei-Ting Hsu, Rigzen P. S. Aulakh, Donald L. Traul, Inn H. Yuk,

Protein purification and stability

In recent years, several automated scale-down bioreactor systems have been developed to increase efficiency in cell culture process development. ambr™ is an automated workstation that provides individual monitoring and control of culture dissolved oxygen and pH in single-use, stirred-tank bioreactors at a working volume of 10-15 mL. To evaluate the ambr™ system, we compared the performance of four recombinant Chinese hamster ovary cell lines in a fed-batch process in parallel ambr™, 2-L bench-top bioreactors, and shake flasks. Cultures in ambr™ matched 2-L bioreactors in controlling the environment (temperature, dissolved oxygen, and pH) and in culture performance (growth, viability, glucose, lactate, Na(+), osmolality, titer, and product quality). However, cultures in shake flasks did not show comparable performance to the ambr™ and 2-L bioreactors.