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Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

2012; Sociedade Brasileira Para o Desenvolvimento da Pesquisa em Cirurgia; Volume: 27; Issue: 8 Linguagem: Inglês

10.1590/s0102-86502012000800003

1678-2674

Conceição Aparecida Dornelas, Francisco Vagnaldo Fechine-Jamacaru, Irineu Lima Albuquerque, Hemerson Iury Ferreira Magalhães, Thiago Alves Dias, M. Faria, Markênia Kely Santos Alves, Sílvia Helena Barem Rabenhorst, Paulo Roberto Carvalho de Almeida, Telma L. G. Lemos, José Daniel Vieira de Castro, Maria Elisabete Amaral de Moraes, Manoel Odorico de Moraes,

Insect and Pesticide Research

To determine the effects of water-soluble derivative of green propolis in bladder cancer angiogenesis in rats given N-butyl-(-4-hydroxybutyl) nitrosamine (BBN).Nine groups were established, where six of them (Groups 1 to 6), the animals received 0.05% BBN in their drinking water for 14 weeks. From the 32nd to the 40th week, Groups 1, 2, 3 and 4 were treated respectively with water, L-lysine (300 mg/kg/day), celecoxib (30 mg/kg/day) and propolis (300 mg/kg/day). Groups 5 and 6 were given propolis and L-lysine from the 1st to the 40th week (150 mg/kg/day). Microvascular density was determined by histological sections stained for the marker CD-31 and analyzed with specific software.The microvascular density in bladder carcinomas was lower (p<0.01) in rats receiving propolis than in controls given carcinogen only. On the other hand, the microvascular density of tumors in rats receiving carcinogen and L-lysine for 40 weeks from the beginning of carcinogen treatment was significantly higher (p<0.01) than in the corresponding controls.Water-soluble derivative of propolis inhibits angiogenesis in BBN-induced rat bladder cancer, while L-lysine treatment stimulates angiogenesis if initiated concurrently with BBN.