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Application of attenuated total reflectance Fourier transform infrared spectrometry to the determination of cephalosporin C in complex fermentation broths

2003; Elsevier BV; Volume: 31; Issue: 3 Linguagem: Inglês

10.1016/s0731-7085(02)00718-5

1873-264X

Kazem Kargosha, Mohammadreza Khanmohammadi, M Sarokhani, Farah Ansari, M Ghadiri,

thermodynamics and calorimetric analyses

An analytical procedure has been developed for quantitative determination of cephalosporin C in complex fermentation broths. The method is based on the partial least-square treatment of data obtained by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectrometric method in the wavenumber range of 1227–1257 cm−1. Absorbance spectra were employed for measurement using a set of eleven binary aqueous standard solutions of cep halosporin C and deacetoxycephalosporin C. The method is simple, rapid and accurate (to within ±1.49%). The developed method has been used to measure cephalosporin C in aqueous solution of biosynthetic samples only after freeze drying the sample at −30 °C and dissolving it in distilled water.