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Near-infrared fluorescent proteins for multicolor in vivo imaging

2013; Nature Portfolio; Volume: 10; Issue: 8 Linguagem: Inglês

10.1038/nmeth.2521

1548-7105

Daria M. Shcherbakova, Vladislav V. Verkhusha,

Photoreceptor and optogenetics research

Four spectrally distinct near-infrared fluorescent proteins based on bacterial phytochromes are described, expanding the possibilities for multicolor in vivo imaging experiments in nontransparent organisms. Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and iRFP720—from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.