Epidermal growth factor receptor and ligands in elongating bovine blastocysts
1998; Wiley; Volume: 51; Issue: 4 Linguagem: Inglês
10.1002/(sici)1098-2795(199812)51
ISSN1098-2795
AutoresAnne Kliem, Frank Tetens, Thomas Klonisch, Maura Grealy, Bernd Fischer,
Tópico(s)Pluripotent Stem Cells Research
ResumoMolecular Reproduction and DevelopmentVolume 51, Issue 4 p. 402-412 Embryo Development Epidermal growth factor receptor and ligands in elongating bovine blastocysts Anne Kliem, Anne Kliem Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle, GermanySearch for more papers by this authorFrank Tetens, Frank Tetens Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle, GermanySearch for more papers by this authorThomas Klonisch, Thomas Klonisch Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle, GermanySearch for more papers by this authorMaura Grealy, Maura Grealy Teagasc Western Research Centre, Belclare, Tuam, County Galway, IrelandSearch for more papers by this authorBernd Fischer, Corresponding Author Bernd Fischer bernd.fischer@medizin.uni-halle.de Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle, GermanyDepartment of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Grosse Steinstrasse 52, D-06097 Halle, Germany.Search for more papers by this author Anne Kliem, Anne Kliem Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle, GermanySearch for more papers by this authorFrank Tetens, Frank Tetens Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle, GermanySearch for more papers by this authorThomas Klonisch, Thomas Klonisch Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle, GermanySearch for more papers by this authorMaura Grealy, Maura Grealy Teagasc Western Research Centre, Belclare, Tuam, County Galway, IrelandSearch for more papers by this authorBernd Fischer, Corresponding Author Bernd Fischer bernd.fischer@medizin.uni-halle.de Department of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Halle, GermanyDepartment of Anatomy and Cell Biology, Martin Luther University Faculty of Medicine, Grosse Steinstrasse 52, D-06097 Halle, Germany.Search for more papers by this author First published: 12 December 1998 https://doi.org/10.1002/(SICI)1098-2795(199812)51:4 3.0.CO;2-9Citations: 25AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Preimplantation development depends on multiple interactions between mother and embryo. The Epidermal Growth Factor Receptor (EGF-R) and its ligands are potential components of the embryo-maternal cross-talk. Employing RT-PCR, in situ hybridization, and immunohistochemistry, we investigated on mRNA and protein level the expression of EGF-R, Epidermal Growth Factor (EGF), Transforming Growth Factor alpha (TGF-α), and Heparin-binding EGF-like Growth Factor (HB-EGF) in spherical and elongating bovine blastocysts between day 13 and day 16 of gestation, and in endometrium at day 13 of gestation. EGF-R mRNA and protein were detected in trophoblast and endoderm cells of all blastocyst stages that were studied, and in luminal and some glandular epithelial cells of the endometrium at day 13. EGF protein was detected in both blastocysts and endometrial epithelium. TGF-α transcripts and protein were present in blastocysts prior to and after elongation and in uterine glandular and luminal epithelium at day 13 of gestation. HB-EGF mRNA and protein was shown in the endoderm, and the protein also was detected immunohistochemically in about 45% of the blastocysts. This presence of the EGF receptor-ligand system in the endometrium and the preimplantation embryo at the time of blastocyst elongation suggests an important role for these growth factors during bovine preimplantation development. Mol. Reprod. Dev. 51:402–412, 1998. © 1998 Wiley-Liss, Inc. Citing Literature Volume51, Issue4December 1998Pages 402-412 RelatedInformation
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