Microtubule Minus-End Stabilization by Polymerization-Driven CAMSAP Deposition
2014; Elsevier BV; Volume: 28; Issue: 3 Linguagem: Inglês
10.1016/j.devcel.2014.01.001
ISSN1878-1551
AutoresKai Jiang, Shasha Hua, Renu Mohan, Ilya Grigoriev, Kah Wai Yau, Qingyang Liu, Eugene A. Katrukha, Maarten Altelaar, Albert J. R. Heck, Casper C. Hoogenraad, Anna Akhmanova,
Tópico(s)Cellular Mechanics and Interactions
ResumoMicrotubules are cytoskeletal polymers with two structurally and functionally distinct ends, the plus- and the minus-end. Here, we focus on the mechanisms underlying the regulation of microtubule minus-ends by the CAMSAP/Nezha/Patronin protein family. We show that CAMSAP2 is required for the proper organization and stabilization of interphase microtubules and directional cell migration. By combining live-cell imaging and in vitro reconstitution of microtubule assembly from purified components with laser microsurgery, we demonstrate that CAMSAPs regulate microtubule minus-end growth and are specifically deposited on the lattice formed by microtubule minus-end polymerization. This process leads to the formation of CAMSAP-decorated microtubule stretches, which are stabilized from both ends and serve as sites of noncentrosomal microtubule outgrowth. The length of the stretches is regulated by the microtubule-severing protein katanin, which interacts with CAMSAPs. Our data thus indicate that microtubule minus-end assembly drives the stabilization of noncentrosomal microtubules and that katanin regulates this process.
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