Solid phase microextraction fills the gap in tissue sampling protocols
2013; Elsevier BV; Volume: 803; Linguagem: Inglês
10.1016/j.aca.2013.08.031
ISSN1873-4324
AutoresBarbara Bojko, Krzysztof Goryński, Germán Augusto Gómez‐Ríos, Jan M. Knaak, Tiago Machuca, Vinzent N. Spetzler, Erasmus Cudjoe, Michael Hsin, Marcelo Cypel, Markus Selzner, Mingyao Liu, Shaf Keshavjee, Janusz Pawliszyn,
Tópico(s)Mass Spectrometry Techniques and Applications
ResumoMetabolomics and biomarkers discovery are an integral part of bioanalysis. However, untargeted tissue analysis remains as the bottleneck of such studies due to the invasiveness of sample collection, as well as the laborious and time-consuming sample preparation protocols. In the current study, technology integrating in vivo sampling, sample preparation and global extraction of metabolites – solid phase microextraction was presented and evaluated during liver and lung transplantation in pig model. Sampling approaches, including selection of the probe, transportation, storage conditions and analyte coverage were discussed. The applicability of the method for metabolomics studies was demonstrated during lung transplantation experiments.
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