Produção Nacional
Revisado por pares
Overexpression of myosin-IIB in the brain of a rat model of streptozotocin-induced diabetes
2011; Elsevier BV; Volume: 303; Issue: 1-2 Linguagem: Inglês
10.1016/j.jns.2011.01.017
ISSN1878-5883
AutoresLuciana Karen Calábria, Gabriel Costa Nunes da Cruz, Rafael Nascimento, Washington João Carvalho, Neire Moura de Gouveia, Fernanda Vieira Alves, Fabiana Barcelos Furtado, Hellen Ishikawa‐Ankerhold, Marcelo Valle de Sousa, Luíz Ricardo Goulart, Foued Salmen Espíndola,
Tópico(s)Signaling Pathways in Disease
ResumoThe Ca2+/calmodulin complex interacts with and regulates various enzymes and target proteins known as calmodulin-binding proteins (CaMBPs). This group of proteins includes molecular motors such as myosins. In this study, we show that non-muscle myosin-IIB is overexpressed in the brains of diabetic rats. We isolated CaMBPs from the brains of non-diabetic rats and rats with streptozotocin-induced diabetes and purified them by immobilized-calmodulin affinity chromatography. The proteins were eluted with EGTA and urea, separated by SDS-PAGE, digested and submitted to peptide mass fingerprinting analysis. Thirteen intense bands were found in both types of brains, two were found exclusively in non-diabetic brains and four were found exclusively in diabetic brains. A large fraction of the eluted proteins contained putative IQ motifs or calmodulin-binding sites. The results of the myosin-IIB affinity chromatography elution, western blot and RT-PCR analyses suggest that myosin-IIB protein and mRNA are expressed at high levels in diabetic brains. This is the first study that has demonstrated differential expression of CaMBPs in diabetic and non-diabetic brain tissue through a comparative proteomic analysis, and it opens up a new approach to studying the relationship between the expression of myosins in the brain, hyperglycemia and intracellular calcium regulation.
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