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Effect of Estrogen on Urethral Function and Nerve Regeneration Following Pudendal Nerve Crush in the Female Rat

2006; Lippincott Williams & Wilkins; Volume: 175; Issue: 5 Linguagem: Inglês

10.1016/s0022-5347(05)00894-3

1527-3792

Yasmin Ahmed, Dan Lin, Corri L. Ferguson, Nereida Esparza, Margot S. Damaser,

Menopause: Health Impacts and Treatments

No AccessJournal of UrologyInvestigative urology1 May 2006Effect of Estrogen on Urethral Function and Nerve Regeneration Following Pudendal Nerve Crush in the Female Rat Yasmin Ahmed, Dan Li Lin, Corri Ferguson, Nereida Esparza, and Margot S. Damaser Yasmin AhmedYasmin Ahmed , Dan Li LinDan Li Lin , Corri FergusonCorri Ferguson , Nereida EsparzaNereida Esparza , and Margot S. DamaserMargot S. Damaser View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)00894-3AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We tested the hypothesis that estrogen promotes improvement in urethral function and nerve regeneration following bilateral pudendal nerve crush in ovariectomized female rats. Materials and Methods: A total of 52 female rats underwent ovariectomy 6 days before bilateral pudendal nerve crush. Estrogen and sham capsules were subcutaneously implanted at the time of nerve crush in 16 and 14 of these rats, respectively, while 22 served as unoperated controls. Seven days following nerve crush urethral LPP testing was performed using urethane anesthesia. Spinal cord sections containing motoneurons of Onufrowicz’s nucleus were subjected to in situ hybridization to detect the expression of βII tubulin mRNA, a marker of the neuroregenerative response. Results: Mean LPP ± SEM was significantly decreased after pudendal nerve crush in sham treated animals compared to unoperated controls (32.1 ± 6.8 vs 54.4 ± 11.6 cm H2O). Rats with an estrogen implant had an LPP of 42.5 ± 16.8 cm H2O, which was significantly greater than rats given sham implants and significantly less than unoperated controls. Rats that received an estrogen implant had increased βII tubulin mRNA expression compared to those that received a sham implant. 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Link, Google Scholar Research Service, Edward Hines, Jr. Veterans Affairs Hospital, Hines and Department of Urology, Loyola University of Chicago Medical Center, Maywood, Illinois© 2006 by American Urological AssociationFiguresReferencesRelatedDetailsCited byHijaz A, Daneshgari F, Sievert K and Damaser M (2018) Animal Models of Female Stress Urinary IncontinenceJournal of Urology, VOL. 179, NO. 6, (2103-2110), Online publication date: 1-Jun-2008. Volume 175Issue 5May 2006Page: 1948-1952 Advertisement Copyright & Permissions© 2006 by American Urological AssociationKeywordsSprague-Dawleyestrogenurinary incontinenceurethranerve crushratsAcknowledgmentsDr. Monica Oblinger, North Chicago Finch School of Medicine, North Chicago, Illinois provided RBT-1 3′UTP. Dr. Kathryn J. Jones provided assistance. Study was performed at Hines Veterans Affairs Hospital and Loyola University School of Medicine.MetricsAuthor Information Yasmin Ahmed More articles by this author Dan Li Lin More articles by this author Corri Ferguson More articles by this author Nereida Esparza More articles by this author Margot S. Damaser Financial interest and/or other relationship with Pfizer. More articles by this author Expand All Advertisement PDF downloadLoading ...