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Nordihydroguaiaretic acid is a potent in vitro scavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion and hypochlorous acid and prevents in vivo ozone-induced tyrosine nitration in lungs

2006; Taylor & Francis; Volume: 40; Issue: 5 Linguagem: Inglês

10.1080/10715760500419365

1071-5762

Esaú Floriano-Sánchez, Esaú Floriano-Sánchez, Cleva Villanueva, Esaú Floriano-Sánchez, Cleva Villanueva, Omar Noel Medina-Campos, Esaú Floriano-Sánchez, Cleva Villanueva, Omar Noel Medina-Campos, Diana Rocha-Mendoza, Esaú Floriano-Sánchez, Cleva Villanueva, Omar Noel Medina-Campos, Diana Rocha-Mendoza, Dolores Javier Sánchez‐González, Esaú Floriano-Sánchez, Cleva Villanueva, Omar Noel Medina-Campos, Diana Rocha-Mendoza, Dolores Javier Sánchez‐González, Noemí Cárdenas‐Rodríguez, José Pedraza‐Chaverrí, Esaú Floriano-Sánchez, Cleva Villanueva, Omar Noel Medina-Campos, Diana Rocha-Mendoza, Dolores Javier Sánchez‐González, Noemí Cárdenas‐Rodríguez, José Pedraza‐Chaverrí,

Nitric Oxide and Endothelin Effects

The antioxidant nordihydroguaiaretic acid (NDGA) has recently become well known as a putative anticancer drug. In this paper, it was evaluated the in vitro peroxynitrite (ONOO− ), singlet oxygen (1O2), hydroxyl radical (OH√), hydrogen peroxide (H2O2), superoxide anion and hypochlorous acid (HOCl) scavenging capacity of NDGA. It was found that NDGA scavenges: (a) ONOO− (IC50 = 4 ± 0.94 μM) as efficiently as uric acid; (b) 1O2 (IC50 = 151 ± 20 μM) more efficiently than dimethyl thiourea, lipoic acid, N-acetyl-cysteine and glutathione; (c) OH√ (IC50 = 0.15 ± 0.02 μM) more efficiently than dimethyl thiourea, uric acid, trolox, dimethyl sulfoxide and mannitol, (d) (IC50 = 15 ± 1 μM) more efficiently than N-acetyl-cysteine, glutathione, tempol and deferoxamine and (e) HOCl (IC50 = 622 ± 42 μM) as efficiently as lipoic acid and N-acetyl-cysteine. NDGA was unable to scavenge H2O2. In an in vivo study in rats, NDGA was able to prevent ozone-induced tyrosine nitration in lungs. It is concluded that NDGA is a potent in vitro scavenger of ONOO− , 1O2, OH√, and HOCl and is able to prevent lung tyrosine nitration in vivo.