Portal de Periódicos da CAPES

Protective Effects of Wu-Zi-Yan-Zong-Fang on Amyloid β-induced Damage <i>In Vivo</i> and <i>In Vitro</i>

2009; Pharmaceutical Society of Japan; Volume: 129; Issue: 8 Linguagem: Inglês

10.1248/yakushi.129.941

1347-5231

Bin Wang, Xue Mei Wang, Hong Fu, Geng Xin Liu,

Tryptophan and brain disorders

This study was designed to determine the effects of Wu-Zi-Yan-Zong-Fang on amyloid-β25-35-induced cognitive deficits in rats and neurotoxicity in pheochromocytoma cells and the possible mechanism of action. In vivo studies showed that Wu-Zi-Yan-Zong-Fang significantly ameliorated the spatial memory and retention deficits, decreased acetylcholinesterase activity, and increased acetylcholine content caused by intracerebroventricular injection of amyloid-β25-35. In vitro results showed that Wu-Zi-Yan-Zong-Fang increased cell viability and the activity of superoxide dismutase and catalase and decreased the release of lactate dehydrogenase and the level of malondialdehyde. Wu-Zi-Yan-Zong-Fang also significantly reduced the percentage of apoptotic cells and blocked the increase in the intracellular concentration of Ca2+. These data suggest that Wu-Zi-Yan-Zong-Fang has potent protective effects for the treatment of Alzheimer's disease in future.