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Hydrogen peroxide‐induced apoptosis in human gastric carcinoma MGC803 cells

2006; Wiley; Volume: 30; Issue: 4 Linguagem: Inglês

10.1016/j.cellbi.2005.12.008

1095-8355

Yubin Mao, Gang Song, Qiufeng Cai, Min Liu, Haohong Luo, Mingxin Shi, Gaoliang Ouyang, Shideng Bao,

MicroRNA in disease regulation

Hydrogen peroxide (H(2)O(2)), a representative ROS, has been used to study the apoptosis of cancer cells to oxidative stress. In this study, we exploited the cellular and molecular mechanisms involved in H(2)O(2)-induced apoptosis in human gastric carcinoma MGC803 cells. Exposure of cells to H(2)O(2) might cause significant viability loss and the increase in apoptotic rate. Treatment with 0.4 mmol/L H(2)O(2) up-regulated Bax but down-regulated Bcl-2 in a time-dependent manner, while Bcl-xL expression remained unchanged. Our results also showed that the levels of Fas and Fas-L were increased, the pro-caspase-3 and pro-caspase-9 were down-regulated in H(2)O(2)-treated MGC803 cells. Under H(2)O(2) stress, we found that the protein p53 also participated in MGC803 cells apoptosis. Taken together, the present study indicated that Fas-mediated cell surface death receptor pathway and mitochondria-mediated pathway may participate in regulating the MGC803 cells apoptosis under oxidative stress.