Portal de Periódicos da CAPES

Benzidine–DNA adduct levels in human peripheral white blood cells significantly correlate with levels in exfoliated urothelial cells

1997; Elsevier BV; Volume: 393; Issue: 3 Linguagem: Inglês

10.1016/s1383-5718(97)00097-1

1879-3592

Qing Zhou, Glenn Talaska, Marlene Jaeger, Vijay Bhatnagar, Richard B. Hayes, Terry V Zenzer, Sri Kant KASHYAP, Vijay M Lakshmi, Rekha Kashyap, Mustafa Dosemeci, Fong‐Fu Hsu, Dinesh J. Parikh, Bernard B. Davis, Nathaniel Rothman,

Carcinogens and Genotoxicity Assessment

In a cross-sectional study of 33 workers exposed to benzidine and benzidine dyes and 15 non-exposed controls, we previously reported that exposure status and internal dose of benzidine metabolites were strongly correlated with the levels of specific benzidine–DNA adducts in exfoliated urothelial cells. We also evaluated DNA adduct levels in peripheral white blood cells (WBC) of a subset of 18 exposed workers and 7 controls selected to represent a wide range of adducts in exfoliated urothelial cells. Samples were coded and then DNA was analyzed using 32P-postlabeling, along with n-butanol extraction. One adduct, which co-chromatographed with a synthetic N-(3-phospho-deoxyguanosin-8-yl)-N′-acetylbenzidine standard, predominated in those samples with adducts present. The median level (range) of this adduct in WBC DNA was 194.4 (3.2–975) RAL×109 in exposed workers and 1.4 (0.1–6.4) in the control subjects (p=0.0002, Wilcoxon Rank Sum Test). There was a striking correlation between WBC and exfoliated urothelial cell adduct levels (Pearson r=0.84, p<0.001) among exposed subjects. In addition, the sum of urinary benzidine, N-acetylbenzidine and N,N′-diacetylbenzidine correlated with the levels of this adduct in both tissues. This is the first study in humans to show a relationship for a specific carcinogen adduct in a surrogate tissue and in urothelial cells, the target for urinary bladder cancer.