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Decoding Human Cytomegalovirus

2012; American Association for the Advancement of Science; Volume: 338; Issue: 6110 Linguagem: Inglês

10.1126/science.1227919

1095-9203

Noam Stern‐Ginossar, Ben Weisburd, Annette Michalski, Vu Thuy Khanh Le‐Trilling, Marco Y. Hein, Sheng‐Xiong Huang, Ming Ma, Ben Shen, Shu‐Bing Qian, Hartmut Hengel, Matthias Mann, Nicholas T. Ingolia, Jonathan S. Weissman,

Advanced biosensing and bioanalysis techniques

Dissecting HCMV Gene Expression Most of us are infected with human cytomegalovirus (HCMV), but severe disease is almost always limited to immunocompromised individuals or newborn infants. The virus has a relatively large (∼240 kb) DNA genome and shows a complex pattern of gene transcription, hinting at a complex regulatory and coding capacity. Stern-Ginossar et al. (p. 1088 ) mapped ribosome positions on HCMV transcripts during the course of viral infection of human fibroblast cells. The data suggest the presence of novel open reading frames (ORFs) lying within existing ORFs; very short ORFs upstream of canonical ORFs; ORFs antisense to canonical ORFs; and short, conserved ORFs encoded by long RNAs. Select ORFs were translated, dramatically expanding the coding capacity of the HCMV genome.