Isolation of an emulsifier from Yarrowia lipolytica NCIM 3589 using a modified mini isoelectric focusing unit
1997; Oxford University Press; Volume: 24; Issue: 2 Linguagem: Inglês
10.1046/j.1472-765x.1997.00355.x
ISSN1472-765X
AutoresSmita Zinjarde, Sathivel Chinnathambi, Anil Lachke, A. Pant,
Tópico(s)Glycosylation and Glycoproteins Research
ResumoLetters in Applied MicrobiologyVolume 24, Issue 2 p. 117-121 Free Access Isolation of an emulsifier from Yarrowia lipolytica NCIM 3589 using a modified mini isoelectric focusing unit S. Zinjarde, S. Zinjarde Division of Biochemical Sciences, National Chemical Laboratory, Pune, IndiaSearch for more papers by this authorS. Chinnathambi, S. Chinnathambi Division of Biochemical Sciences, National Chemical Laboratory, Pune, IndiaSearch for more papers by this authorA. H. Lachke, A. H. Lachke Division of Biochemical Sciences, National Chemical Laboratory, Pune, IndiaSearch for more papers by this authorA. Pant, A. Pant Division of Biochemical Sciences, National Chemical Laboratory, Pune, IndiaSearch for more papers by this author S. Zinjarde, S. Zinjarde Division of Biochemical Sciences, National Chemical Laboratory, Pune, IndiaSearch for more papers by this authorS. Chinnathambi, S. Chinnathambi Division of Biochemical Sciences, National Chemical Laboratory, Pune, IndiaSearch for more papers by this authorA. H. Lachke, A. H. Lachke Division of Biochemical Sciences, National Chemical Laboratory, Pune, IndiaSearch for more papers by this authorA. Pant, A. Pant Division of Biochemical Sciences, National Chemical Laboratory, Pune, IndiaSearch for more papers by this author First published: 31 October 2003 https://doi.org/10.1046/j.1472-765X.1997.00355.xCitations: 31 Dr A. Pant Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract A Yarrowia lipolytica strain (NCIM 3589) isolated in our laboratory produced an emulsifier during the stationary phase when grown in a defined artificial sea water medium containing 1% (v/v) n-hexadecane, as the sole carbon source. The emulsifier was isolated by ultrafiltration, Sepharose 4B followed by isoelectric focusing (IEF) in a miniscale unit in the pH range of 3·0–10 and 3·5–5·0. The pI of the emulsifier was 4·0. The emulsifier is a glycolipid consisting of 5% protein, 20% carbohydrate and 75% lipid. The fatty acid, sugar and amino acid composition of the isolated emulsifier are described along with temperature stability, pH stability and stability in sodium chloride. This paper is a first report on rapid and simple isolation by IEF of a microbial emulsifier. Citing Literature Volume24, Issue2February 1997Pages 117-121 RelatedInformation
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