Metabolic radiolabeling techniques for identification of prenylated and fatty acylated proteins
1990; Elsevier BV; Volume: 1; Issue: 3 Linguagem: Inglês
10.1016/s1046-2023(05)80320-2
ISSN1095-9130
AutoresSusanne M. Mumby, Janice E. Buss,
Tópico(s)Fatty Acid Research and Health
ResumoLipid modifications of proteins can be detected by incubation of cultured cells with radioactive precursors followed by detergent extraction of protein and analysis of the extract by SDS-PAGE and fluorography. Identification of particular proteins requires immunoprecipitation, affinity purification, or differential expression (such as overexpression) before SDS-PAGE analysis. The purpose of this article is to present a brief description of protein acylation and prenylation modifications that have been observed and the details of how to detect the modifications by metabolic radiolabeling of cellular proteins.
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