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Structure characterisation of a α β-glucan polysaccharide from Auricularia polytricha

2011; Taylor & Francis; Volume: 26; Issue: 21 Linguagem: Inglês

10.1080/14786419.2011.633080

1478-6427

Guanglei Song, Qizhen Du,

Microbial Metabolites in Food Biotechnology

Abstract A water-soluble α β-glucan with a molecular weight of 1.62 × 105 Da, AAPS -1, was obtained from the fruiting bodies of the edible mushroom Auricularia polytricha by a combined separation of high-speed countercurrent chromatography (HSCCC)-Sephacryl S-300 HR column chromatography. The structure of AAPS-1 was elucidated that the polysaccharide possesses a backbone composed of (1 → 6)-linked-α-D-glucopyranosyl and (1 → 6)-linked-β-D-glucopyranosyl residues, partially substituted at O-3 of β-D-glucopyranosyl residue by side chain (1 → 4)-α-D-Glcp and terminated with non-reducing end α-D-Glcp-(1 → on the basis of the analyses of partial hydrolysis, periodate oxidation, acetylation, methylation and NMR spectroscopy (1H, 13C). Keywords: polysaccharidechemical structuremushroom Auricularia polytricha Acknowledgements This work was supported by a grant (no. 2007AA10Z342) from the National High Technology Research and Development Program of China (863 Program).