Structure of rhamnan sulfate from the green alga Monostroma nitidum and its anti-herpetic effect
2010; Elsevier BV; Volume: 81; Issue: 3 Linguagem: Inglês
10.1016/j.carbpol.2010.03.014
ISSN1879-1344
AutoresJung‐Bum Lee, Seiji Koizumi, Kyoko Hayashi, Toshimitsu Hayashi,
Tópico(s)Echinoderm biology and ecology
Resumo"A sulfated polysaccharide from Monostroma nitidum Wittrock (Ulvophyceae)" was purified by anion-exchange and gel filtration column chromatographies. The isolated polysaccharide consisted of large amount of l-rhamnose with small amount of d-glucose, and it was regarded to be a rhamnan sulfate (RS). Methylation analysis of the native and desulfated polysaccharide suggested that this polymer was mainly composed of 1,2- and 1,3-linked rhamnose residues with a ratio of ca 1:2. In addition, it was found the presence of 1,2,3-linked rhamnose and 1,4-linked glucose residues. Sulfate groups were suggested to be mainly located at C-2 and C-3 of 1,3- and 1,2-linked rhamnose residues, respectively. NMR analyses including 1D and 2D experiments indicated that RS consisted of sugar linkage units as follows: 1: →3)-α-l-Rha-(1 → 3)-α-l-Rha-(1→ 2: →3)-α-l-Rha-(1 → 2)-α-l-Rha-(1→ 3: →3)-α-l-Rha-(1 → 3)-α-l-Rha-(1→2β-D-Glc-(1-↑ Moreover, RS showed potent antiviral activity against herpes simplex virus type 2 virus, whereas it has no effect on the replication of influenza A virus. Anti-HSV-2 target(s) of RS was suggested to be virus adsorption and penetration steps onto host cell surface.
Referência(s)