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Cre/loxP System for Generating Tissue-specific Knockout Mouse Models

2004; Oxford University Press; Volume: 62; Issue: 6 Linguagem: Inglês

10.1301/nr2004.jun243-246

1753-4887

Claudine H. Kos,

3D Printing in Biomedical Research

Alteration of the mouse genome by conventional transgenic and gene-targeted approaches has greatly facilitated studies of gene function. However, a gene alteration expressed in the germ line may cause an embryonic lethal phenotype resulting in no viable mouse to study gene function. Similarly, a gene alteration may exert its effect in multiple different cell and tissue types, creating a complex phenotype in which it is difficult to distinguish direct function in a particular tissue from secondary effects resulting from altered gene function in other tissues. Therefore, methods have been developed to control conditions such as the timing, cell-type, and tissue specificity of gene activation or repression. This brief review provides an overview of the Cre/LoxP system for generating tissue-specific knockout mouse models.