Thymus deoxyribonucleoprotein I. Preparation and thermal denaturation
1962; Elsevier BV; Volume: 55; Issue: 6 Linguagem: Inglês
10.1016/0006-3002(62)90907-1
ISSN1878-2434
AutoresKenneth Murray, Arthur Peacocke,
Tópico(s)RNA and protein synthesis mechanisms
ResumoPreparations of undissociated deoxyribonucleoprotein having a reproducible composition were obtained from calf-thymus glands essentially by extraction with water. Spectrophotometric titration and other ultraviolet-absorption measurements showed that this deoxyribonucleoprotein was denatured by heat and by alkali in both 0.001 M and 1.0 M sodium chloride in a manner similar to DNA. This similarity confirms that the double-helical structure of DNA exists within the original undissociated deoxyribonucleoprotein complex in solutions.
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