Development of a LC/MS/MS method for the analysis of cannabinoids in human EDTA-plasma and urine after small doses of Cannabis sativa extracts
2007; Elsevier BV; Volume: 850; Issue: 1-2 Linguagem: Inglês
10.1016/j.jchromb.2006.12.045
ISSN1873-376X
AutoresSandra Béatrice Grauwiler, André Scholer, Jürgen Drewe,
Tópico(s)Alcohol Consumption and Health Effects
ResumoA novel high-performance liquid chromatographic separation method with tandem-mass spectrometry detection was developed for the simultaneous determination of Δ9-tetrahydrocannabinol (THC) and its major metabolites 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC) and 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) as well as the components cannabidiol (CBD) and cannabinol (CBN) in human EDTA-plasma and urine. Run time was 25 min. Lower limit of quantification was 0.2 ng/ml. The coefficients of variation of all inter- and intra-assay determinations were between 1.3 and 15.5%. The method was successfully applied to the determination of cannabinoids in human plasma and human urine after administration of Δ9-tetrahydrocannabinol or Cannabis sativa extracts.
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