Thermococcus stetteri sp. nov., a New Extremely Thermophilic Marine Sulfur-Metabolizing Archaebacterium
1989; Elsevier BV; Volume: 12; Issue: 3 Linguagem: Inglês
10.1016/s0723-2020(89)80071-2
ISSN1618-0984
AutoresM. L. Miroshnichenko, E. A. Bonch-Osmolovskaya, A. Neuner, N. A. Kostrikina, N.A. Chernych, V.A. Alekseev,
Tópico(s)Methane Hydrates and Related Phenomena
ResumoFour strains of new extremely thermophilic anaerobic archaebacteria were isolated from marine solfataric fields of Kraternaya cove (Ushishir archipelago, Northern Kurils). The cells are irregular cocci 1 to 2 μm in diameter. Two strains are motile due to a tuft of flagella. Two strains are non-motile. The cell envelope consists of two layers of subunits. Two strains (non-motile) grow at temperatures from 55 to 94°C (opt. 75 °C) and two (motile) from 75 to 98 °C. The pH range for growth of all strains is 5.7 to 7.2 (opt. around 6.5). Salt (opt. 2.5% NaCl) and elemental sulfur are obligately required for growth. Peptides and polysaccharides are utilized. During growth more than 20 μmol/ml H2S are formed and CO2, acetate, isobutyrate and isovalerate are produced. The G+C content of DNA of isolate K-3 is 50.2 mol.%. The four isolates exhibited high DNA homology among each other, indicating that they belong to the same species. Partial 16S rRNA sequencing of isolate K-3 indicated that it belongs to the genus Thermococcus. It shows no significant DNA homology with Thermococcus celer. In view of all differences between the isolates and Thermococcus celer, they are considered as representatives of a new species described as Thermococcus stetteri. Type strain is isolate K-3, DSM 5262.
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