High-performance liquid chromatography and ultrafiltration of whey proteins with inorganic porous materials coated with polyvinylimidazole derivatives
1991; Elsevier BV; Volume: 548; Linguagem: Inglês
10.1016/s0021-9673(01)88603-9
ISSN1873-3778
AutoresBernard Chaufer, Marianne Rollin, Bernard Sébille,
Tópico(s)Electrostatics and Colloid Interactions
ResumoAbstract A coated ultrafiltration membrane was developed which allows the highly selective extraction of lactalbumin from a whey protein mixture. A permeate containing only lactalbumin was obtained using inorganic membranes coated with polyvinylimidazole derivatives containing both ionic and hydrophobic groups. The phenomena involved were analysed with high-performance liquid chromatographic supports bearing similar coatings. With the hydrophobic layer, the selectivity enhancement can be explained through mixed interactions with whey proteins; increasing fouling is due to both lactoglobulin and lactalbumin contributions to the build-up of the boundary layer at the wall of the derivatized membrane.
Referência(s)