Produção Nacional
Revisado por pares
Comparison Between the Effect of Low-Level Laser Therapy and Low-Intensity Pulsed Ultrasonic Irradiation in Vitro
2008; Mary Ann Liebert, Inc.; Volume: 26; Issue: 1 Linguagem: Inglês
10.1089/pho.2007.2112
ISSN1557-8550
AutoresRodrigo Franco de Oliveira, Deise A. A. Pires Oliveira, Wagner Monteiro, Renato Amaro Zângaro, Márcio Magini, Cristina Pacheco Soares,
Tópico(s)Tendon Structure and Treatment
ResumoObjective: The objective of this study was to compare the effect of low-level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS) on fibroblast cell culture. Several methods, including ultrasound treatment and LLLT, are being used to facilitate tissue repair and healing processes. Materials and Methods: L929 fibroblast cell cultures were irradiated with low-level laser energy and LIPUS. Cultures irradiated with ultrasound were divided into five groups: group 1: control (did not receive irradiation); group 2: 0.2 W/cm2 in pulsed mode at 10% (1:9 duty cycle); group 3: 0.6 W/cm2 in pulsed mode at 10% (1:9 duty cycle); group 4: 0.2 W/cm2 in pulsed mode at 20% (2:8 duty cycle); and group 5: 0.6 W/cm2 in pulsed mode at 20% (2:8 duty cycle). Cultures irradiated with laser energy were divided into three groups: group 1: control (did not receive irradiation); group 2: 6 J/cm2; and group 3: 50 mJ/cm2. Each group was irradiated at 24-h intervals, with the following incubation periods post-irradiation: 24, 48, and 72 h; after each irradiation cycle the cultures were analyzed using MTT [3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide]. Results: Analysis of results after LLLT and LIPUS demonstrated that the effect of laser therapy on fibroblast cell culture was greater than that of LIPUS (p < 0.05). Conclusion: Results demonstrated that LLLT significantly increased fibroblastic activity more than LIPUS. Therefore, in the first and second phases of tissue repair, laser treatment may be more effective than ultrasound treatment.
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