Fluorometric determination of hydrogen peroxide in milk by using a Fenton reaction system
2009; Elsevier BV; Volume: 120; Issue: 1 Linguagem: Inglês
10.1016/j.foodchem.2009.10.024
ISSN1873-7072
AutoresMoussa Abbas, Wei Luo, Li Zhu, Jing Zou, Heqing Tang,
Tópico(s)Sulfur Compounds in Biology
ResumoA simple and highly sensitive fluorometric method is proposed for the determination of H2O2 in milk samples. In this method, non-fluorescent coumarin is oxidised to highly fluorescent 7-hydroxycoumarin by hydroxyl radicals (OH) generated in a Fenton reaction, and the oxidation product has strong fluorescence with a maximum intensity at 456 nm and can be used as a fluorescence probe for H2O2. Under the optimal conditions (2.5 × 10−4 mol L−1 iron(II) ions, 4.0 × 10−4 mol L−1 coumarin, solution pH 3.0, reaction time 9 min, and excitation at 346 nm), the proposed method presents wide linear responses between the fluorescence intensity and H2O2 concentration in a wide range from 2.0 × 10−8 to 2.0 × 10−5 mol L−1, with a detection limit (S/N = 3) of 5.0 × 10−9 mol L−1. After possible interferences are evaluated for a series of chemical substances, the present method has been applied to the determination of hydrogen peroxide in milk with satisfactory results.
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