Coexistence of acetylcholinesterase-, human growth hormone-releasing factor(1–37)-, α-melanotropin- and melanin-concentrating hormone-like immunoreactivities in neurons of the rat hypothalamus: a light and electron microscope study
1989; Elsevier BV; Volume: 100; Issue: 1-3 Linguagem: Inglês
10.1016/0304-3940(89)90654-x
ISSN1872-7972
AutoresPierre‐Yves Risold, D Fellmann, D Lenys, C Bugnon,
Tópico(s)Retinoids in leukemia and cellular processes
ResumoUsing an antiserum (AS) raised against rat cerebral acetylcholinesterase (AChE), we revealed a neuron population in lateral and dorsal areas of the posterior rat hypothalamus. These neurons were previously described using antibodies to human growth hormone-releasing factor(1-37) (GRF-37), alpha-melanotropin (alpha-MSH) and melanin-concentrating hormone (MCH). Different intracytoplasmic distributions of the immunodeposits were observed depending on the used serum. Ultrastructural investigations demonstrated that AChE-AS labeled rough endoplasmic reticulum and nuclear envelope in control rats. MCH-AS stained Golgi apparatus in control animals and secretory granules in colchicine-injected rats. GRF-37-AS always revealed secretory granules, and alpha-MSH-AS gave the same staining only after colchicine injection.
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