Identification of a Gene Encoding a Hyperpolarization-Activated Pacemaker Channel of Brain
1998; Cell Press; Volume: 93; Issue: 5 Linguagem: Inglês
10.1016/s0092-8674(00)81434-8
ISSN1097-4172
AutoresBina Santoro, David T. Liu, Huan Yao, Dušan Bartsch, Eric R. Kandel, Steven A. Siegelbaum, Gareth R. Tibbs,
Tópico(s)Cardiac electrophysiology and arrhythmias
ResumoThe generation of pacemaker activity in heart and brain is mediated by hyperpolarization-activated cation channels that are directly regulated by cyclic nucleotides. We previously cloned a novel member of the voltage-gated K channel family from mouse brain (mBCNG-1) that contained a carboxy-terminal cyclic nucleotide-binding domain (39Santoro B Grant S.G.N Bartsch D Kandel E.R Interactive cloning with the SH3 domain of N-src identifies a new brain specific ion channel protein, with homology to eag and cyclic nucleotide-gated channels.Proc. Natl. Acad. Sci. USA. 1997; 94: 14815-14820Crossref PubMed Scopus (224) Google Scholar) and hence proposed it to be a candidate gene for pacemaker channels. Heterologous expression of mBCNG-1 demonstrates that it does indeed code for a channel with properties indistinguishable from pacemaker channels in brain and similar to those in heart. Three additional mouse genes and two human genes closely related to mBCNG-1 display unique patterns of mRNA expression in different tissues, including brain and heart, demonstrating that these channels constitute a widely expressed gene family.
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