Portal de Periódicos da CAPES

Rapid analysis of transgenic trees using transmittance near-infrared spectroscopy (NIR)

2006; De Gruyter; Volume: 60; Issue: 1 Linguagem: Inglês

10.1515/hf.2006.005

1437-434X

Tatsuhiko Yamada, Ting‐Feng Yeh, Hou‐min Chang, Laigeng Li, John F. Kadla, Vincent L. Chiang,

Oil Palm Production and Sustainability

Abstract Genetic engineering of trees has generated a large amount of interest in the development of highly improved transgenic trees. To efficiently monitor and control the properties of the transgenic products, a rapid, mini-scale analytical method is required. Transmittance near-infrared (NIR) spectroscopy was chosen as a fast analysis tool for characterizing the chemical properties of the transgenic products. Pellets were prepared from 75 mg of wood meal and directly scanned using transmittance NIR spectroscopy. Very strong correlations were obtained between the NIR data and conventional wet-chemistry results for the lignin content, S/G ratio, cellulose and xylose content. The results indicate that transmittance NIR is a powerful tool for determining and screening the chemical properties of transgenic trees.