Expression of the c-kit Proto-Oncogene and Its Ligand Stem Cell Factor (SCF) in Normal and Malignant Human Testicular Tissue
1995; Lippincott Williams & Wilkins; Volume: 153; Issue: 2 Linguagem: Inglês
10.1097/00005392-199502000-00073
ISSN1527-3792
AutoresT. Strohmeyer, David Reese, Michael F. Press, R. Ackermann, Michael Hartmann, Dennis J. Slamon,
Tópico(s)Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
ResumoNo AccessJournal of UrologyInvestigative Urology1 Feb 1995Expression of the c-kit Proto-Oncogene and Its Ligand Stem Cell Factor (SCF) in Normal and Malignant Human Testicular Tissue Torsten Strohmeyer, David Reese, Michael Press, Rolf Ackermann, Michael Hartmann, and Dennis Slamon Torsten StrohmeyerTorsten Strohmeyer , David ReeseDavid Reese , Michael PressMichael Press , Rolf AckermannRolf Ackermann , Michael HartmannMichael Hartmann , and Dennis SlamonDennis Slamon View All Author Informationhttps://doi.org/10.1097/00005392-199502000-00073AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Recent findings suggest an important role of the proto-oncogene c-kit, a surface membrane receptor of the tyrosine kinase family, and its ligand stem cell factor (SCF) in normal spermatogenesis and possibly in the pathogenesis of certain testicular germ cell tumors. To further investigate this potential role, the expression of c-kit and SCF was studied in normal and malignant human testicular tissue specimens at the mRNA and protein level by Northern blot analysis and immunohistochemistry, respectively. The detection of the c-kit receptor in normal human germ cells and its natural ligand SCF in Sertoli cells suggests the presence of a local trophic regulatory system that may be active in human spermatogenesis. Additionally, c-kit expression was detected in the seminoma but not in the nonseminoma subtype of human testicular germ cell tumors (GCT). Stem cell factor was not expressed at the mRNA level in tissue from either subtype of GCT as determined by Northern blot analysis; however, the protein was detected immunohistochemically in the cytoplasm of rare tumor cells. References 1 : Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. E.M.B.O. J.1987; 6: 3341. 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School of Medicine, Los Angeles, California, Department of Urology, Heinrich-Heine-University of Dusseldorf, Dusseldorf, Germany, Department of Pathology, USC Medical Center, Los Angeles, California, Department of Urology, Army Hospital Hamburg, Hamburg, GermanyRequests for reprints: Department of Oncology, Schering AG, 13342 Berlin, Germany© 1995 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited bySchmidt B, Ackermann R, Hartmann M and Strohmeyer T (2018) ALTERATIONS OF THE METASTASIS SUPPRESSOR GENE nm23 AND THE PROTO-ONCOGENE c-myc IN HUMAN TESTICULAR GERM CELL TUMORSJournal of Urology, VOL. 158, NO. 5, (2000-2005), Online publication date: 1-Nov-1997.Strohmeyer D, Langenhof S, Ackermann R, Hartmann M, Strohmeyer T and Schmidt B (2018) Analysis of the DCC Tumor Suppressor Gene in Testicular Germ Cell Tumors: Mutations and Loss of ExpressionJournal of Urology, VOL. 157, NO. 5, (1973-1976), Online publication date: 1-May-1997. Volume 153Issue 2February 1995Page: 511-515 Advertisement Copyright & Permissions© 1995 by American Urological Association, Inc.MetricsAuthor Information Torsten Strohmeyer More articles by this author David Reese More articles by this author Michael Press More articles by this author Rolf Ackermann More articles by this author Michael Hartmann More articles by this author Dennis Slamon More articles by this author Expand All Advertisement PDF downloadLoading ...
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