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Physical anchorage and orientation of equine linkage groups by FISH mapping BAC clones containing microsatellite markers

2001; Wiley; Volume: 32; Issue: 1 Linguagem: Inglês

10.1046/j.1365-2052.2001.00715.x

1365-2052

Gabriella Lindgren, June Swinburne, Matthew Breen, Denis Mariat, Kaj Sandberg, G. Guérin, Hans Ellegren, M. M. Binns,

Genomics and Phylogenetic Studies

A horse bacterial artificial chromosome (BAC) library was screened for 19 microsatellite markers from unassigned or non‐oriented linkage groups. Clones containing 11 ( AHT20 , EB2E8 , HMS45 , LEX005 , LEX014 , LEX023 , LEX044 , TKY111 , UCDEQ425 , UCDEQ464 and VIASH21 ) of these were found, which were from eight different linkage groups. The BAC clones were used as probes in dual colour FISH to identify their precise chromosomal origin. The microsatellite markers are located on nine different horse chromosomes, four of which (ECA6, ECA25, ECA27 and ECA28) had no previously in situ assigned markers.