Portal de Periódicos da CAPES

Robotic production of 2-deoxy-2[18F]fluoro-d-glucose: A routine method of synthesis using tetrabutylammonium [18F]fluoride

1988; Pergamon Press; Volume: 39; Issue: 7 Linguagem: Inglês

10.1016/0883-2889(88)90060-3

1878-1128

James W. Brodack, Carmen S. Dence, Michael R. Kilbourn, Michael J. Welch,

Cancer, Hypoxia, and Metabolism

Using existing robotic hardware and software programs developed for the synthesis of several positron-emitting radiopharmaceuticals for PET imaging [Brodack et al. (1988) Appl. Radiat. Isot.39, 689], the additional automated synthesis of 2-deoxy-2-[18F]fluoro-d-glucose (2-[18F]FDG) has been incorporated into our Zymate Laboratory Automation System. The robotic synthesis of 2-[18F]FDG took less than one week to implement, including the organization of software subroutines and construction of an additional heating station. The end of synthesis yield (12–17%) and radiochemical purity (96–99%) for the robotic preparation of 2-[18F]FDG is similar to that of the manual synthesis. This automated method uses anhydrous tetrabutylammonium [18F]fluoride as the reactive fluoride source in the labeling step. The procedure is a modification of the synthesis reported by Hamacher et al. [Hamacher et al. (1986) J. Nucl. Med.27, 235].