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Metabonomics: the use of electrospray mass spectrometry coupled to reversed‐phase liquid chromatography shows potential for the screening of rat urine in drug development

2002; Wiley; Volume: 16; Issue: 20 Linguagem: Inglês

10.1002/rcm.813

1097-0231

Robert S. Plumb, Chris L. Stumpf, M. V. Gorenstein, José Castro‐Perez, Gordon J. Dear, Maria L. Anthony, Brian C. Sweatman, Susan C. Connor, John N. Haselden,

Mass Spectrometry Techniques and Applications

The application of liquid chromatography/mass spectrometry (LC/MS) followed by principal components analysis (PCA) has been successfully applied to the screening of rat urine following the administration of three candidate pharmaceuticals. With this methodology it was possible to differentiate the control samples from the dosed samples and to identify the components of the mass spectrum responsible for the separation. These data clearly show that LC/MS is a viable alternative, or complementary, technique to proton NMR for metabonomics applications in drug discovery and development.