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Isolation and characterization of two sub-species of Lp(a), one containing apo E and one free of apo E

1992; Elsevier BV; Volume: 1127; Issue: 2 Linguagem: Inglês

10.1016/0005-2760(92)90267-y

1879-145X

Jean‐Marie Bard, Sophie Lestavel, Véronique Clavey, Pascal Pont, Bruno Derudas, Henri‐Joseph Parra, Jean‐Charles Fruchart,

Cholesterol and Lipid Metabolism

Lipoprotein Lp(a) was isolated by immunoaffinity chromatography using anti apolipoprotein B and anti apolipoprotein (a) immunosorbents. Besides apolipoproteins (a) and B, this fraction was shown to contain apolipoproteins C and E. Therefore, it was decided to further purify this crude Lp(a) into particles containing apolipoprotein E and particles free of apo E, using chromatography with an anti apolipoprotein E immuosorbent. Lp(a), free of apolipoprotein E was cholesterol ester rich and triacylglycerol poor and was found mainly in the LDL size range. In contrast, Lp(a) containing apolipoprotein E was triacylglycerol rich and was distributed mainly in the VLDL nd IDL size range. Binding of these two fractions, one containing apo E and one free of it, to the apo B/E receptor of HeLa cells was studied. Both fractions bound to the receptor but the one containing apo E had a better affinity than the one free of apo E. Further studies are needed to identity the clinical importance of these two different entities.