Promoter 1 ofLMO2, a master gene for hematopoiesis, is regulated by the erythroid specific transcription factor GATA1
2000; Wiley; Volume: 1; Issue: 2 Linguagem: Inglês
10.1002/1438-826x(200009)1
ISSN1438-826X
AutoresMaik Martin Pruess, Matthias Drechsler, Brigitte Royer‐Pokora,
Tópico(s)Hemoglobinopathies and Related Disorders
ResumoGene Function & DiseaseVolume 1, Issue 2 p. 87-94 Research Paper Promoter 1 of LMO2, a master gene for hematopoiesis, is regulated by the erythroid specific transcription factor GATA1 Maik Martin Pruess, Maik Martin Pruess Institute of Human Genetics and Anthropology, Heinrich-Heine University, Duesseldorf, Germany Max-Planck Institute for Molecular Genetics, Berlin, GermanySearch for more papers by this authorMatthias Drechsler, Matthias Drechsler Institute of Human Genetics and Anthropology, Heinrich-Heine University, Duesseldorf, GermanySearch for more papers by this authorBrigitte Royer-Pokora, Corresponding Author Brigitte Royer-Pokora [email protected]. Institute of Human Genetics and Anthropology, Heinrich-Heine University, Duesseldorf, GermanyHeinrich-Heine University, Institute of Human Genetics and Anthropology, Postfach 101007, D-40001 Duesseldorf, Germany. Tel. +49- 211−81−12350; Fax +49−211−81−12538Search for more papers by this author Maik Martin Pruess, Maik Martin Pruess Institute of Human Genetics and Anthropology, Heinrich-Heine University, Duesseldorf, Germany Max-Planck Institute for Molecular Genetics, Berlin, GermanySearch for more papers by this authorMatthias Drechsler, Matthias Drechsler Institute of Human Genetics and Anthropology, Heinrich-Heine University, Duesseldorf, GermanySearch for more papers by this authorBrigitte Royer-Pokora, Corresponding Author Brigitte Royer-Pokora [email protected]. Institute of Human Genetics and Anthropology, Heinrich-Heine University, Duesseldorf, GermanyHeinrich-Heine University, Institute of Human Genetics and Anthropology, Postfach 101007, D-40001 Duesseldorf, Germany. Tel. +49- 211−81−12350; Fax +49−211−81−12538Search for more papers by this author First published: 17 August 2001 https://doi.org/10.1002/1438-826X(200009)1:2 3.0.CO;2-4Citations: 6AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The T cell oncogene LMO2 was first identified at the site of the translocation t(11;14)(p13;q11) in T-acute lymphocytic leukemia (T-ALL) and encodes a cysteine-rich protein with LIM-motifs. It was later shown to have an essential role in yolk sac and adult erythropoiesis. LMO2 encodes two alternative transcripts differing in the length of the 5’ untranslated region, but encoding the same protein. Transcription start site mapping revealed the 5′-end of the longer transcript, LMO2a and promoter 1. Sequencing identified two putative GATA1 sites and an overlapping SP1 site close to the transcription start site, suggesting that promoter 1 (P1) is an erythroid specific promoter. Using EMSA analysis with an oligonucleotide from promoter 1 we now show that GATA1 and SP1 bind to these sites. DNaseI hypersensitive site (DHS) mapping upstream of the transcription start site revealed four erythroid specific sites, corresponding to putative GATA1 motifs and one non-lineage specific site. Reporter gene experiments with P1 and a mutant, where both GATA sites were inactivated, showed that GATA1 plays a functional role in the erythroid specific transcriptional control of LMO2 from P1. 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