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Real-time measurement of fluorescence spectra from single airborne biological particles

1999; Wiley; Volume: 3; Issue: 4-5 Linguagem: Inglês

10.1002/(sici)1520-6521(1999)3

1520-6521

Steven C. Hill, Ronald G. Pinnick, Stanley Niles, Yong‐Le Pan, Stephen Holler, Richard K. Chang, Jerold R. Bottiger, Bean T. Chen, Chun‐Sing Orr, Greg Feather,

Air Quality Monitoring and Forecasting

Field Analytical Chemistry & TechnologyVolume 3, Issue 4-5 p. 221-239 Real-time measurement of fluorescence spectra from single airborne biological particles Steven C. Hill, Corresponding Author Steven C. Hill U. S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783U. S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783Search for more papers by this authorRonald G. Pinnick, Ronald G. Pinnick U. S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783Search for more papers by this authorStanley Niles, Stanley Niles U. S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783Search for more papers by this authorYong-Le Pan, Yong-Le Pan Physical Sciences Laboratory, New Mexico State University, Las Cruces, New Mexico 88003Search for more papers by this authorStephen Holler, Stephen Holler Applied Physics, Yale University, New Haven, Connecticut 06520Search for more papers by this authorRichard K. Chang, Richard K. Chang Applied Physics, Yale University, New Haven, Connecticut 06520Search for more papers by this authorJerold Bottiger, Jerold Bottiger U. S. Army Edgewood Chemical and Biological Center, Aberdeen Proving Ground, Maryland 21010Search for more papers by this authorBean T. Chen, Bean T. Chen National Institute of Occupational Safety and Health, Morgantown, West Virginia 26505Search for more papers by this authorChun-Sing Orr, Chun-Sing Orr National Institute of Occupational Safety and Health, Morgantown, West Virginia 26505Search for more papers by this authorGreg Feather, Greg Feather National Institute of Occupational Safety and Health, Morgantown, West Virginia 26505Search for more papers by this author Steven C. Hill, Corresponding Author Steven C. Hill U. S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783U. S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783Search for more papers by this authorRonald G. Pinnick, Ronald G. Pinnick U. S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783Search for more papers by this authorStanley Niles, Stanley Niles U. S. Army Research Laboratory, 2800 Powder Mill Road, Adelphi, Maryland 20783Search for more papers by this authorYong-Le Pan, Yong-Le Pan Physical Sciences Laboratory, New Mexico State University, Las Cruces, New Mexico 88003Search for more papers by this authorStephen Holler, Stephen Holler Applied Physics, Yale University, New Haven, Connecticut 06520Search for more papers by this authorRichard K. Chang, Richard K. Chang Applied Physics, Yale University, New Haven, Connecticut 06520Search for more papers by this authorJerold Bottiger, Jerold Bottiger U. S. Army Edgewood Chemical and Biological Center, Aberdeen Proving Ground, Maryland 21010Search for more papers by this authorBean T. Chen, Bean T. Chen National Institute of Occupational Safety and Health, Morgantown, West Virginia 26505Search for more papers by this authorChun-Sing Orr, Chun-Sing Orr National Institute of Occupational Safety and Health, Morgantown, West Virginia 26505Search for more papers by this authorGreg Feather, Greg Feather National Institute of Occupational Safety and Health, Morgantown, West Virginia 26505Search for more papers by this author First published: 19 October 1999 https://doi.org/10.1002/(SICI)1520-6521(1999)3:4/5 3.0.CO;2-7Citations: 139AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Improved real-time methods for characterizing airborne biological particles are needed. Here we review our efforts in developing techniques for measuring the laser-induced fluorescence (total and spectrally dispersed) of individual airborne particles, and describe our present system, which can measure fluorescence spectra of single micrometer-sized bioaerosol particles with good signal-to-noise ratios. We demonstrate the capability of this system by showing measured spectra of a variety of airborne particles generated in the laboratory from road dust, ammonium sulfate, Bacillus subtilis and other bacteria prepared under various conditions, allergens, cigarette smoke, and chicken-house dust. These spectra illustrate the capability of the system to distinguish between some biological and nonbiological aerosols, and among several types of laboratory-generated biological aerosols. We suggest improvements needed to make our system field portable. © 1999 John Wiley & Sons, Inc.* Field Analyt Chem Technol 3: 221–239, 1999 References 1 Lighthart B, Shaffer, B. Airborne bacteria in the atmospheric surface layer: Temporal distribution above a grass seed field. Appl Environ Microbiol 1995; 61: 1492–1496. CASWeb of Science®Google Scholar 2 Lighthart B, Stetzenbach LD. Distribution of microbial bioaerosol. In: B Lighthart, AJ Mohr, editors. Atmospheric microbial aerosols. New York: Chapman & Hall; 1994. chap. 4, p 68–98. 10.1007/978-1-4684-6438-2_4 Google Scholar 3 Matthias-Maser, S, Jaenicke, R. Examination of atmospheric bioaerosol particles with radii greater than 0.2 micrometers. J Aerosol Sci 1994; 25: 1605–1613. 10.1016/0021-8502(94)90228-3 CASWeb of Science®Google Scholar 4 Crook B, Olenchock SA. Industrial workplaces. In: CS Cox, CM Wathes, editors. Bioaerosols handbook. Boca Raton: Lewis; 1995. chap 19. Google Scholar 5 Burge H. Bioaerosols in the residential environment. In: CS Cox, CM Wathes, editors. Bioaerosols handbook. Boca Raton: Lewis; 1995. chap. 21. Google Scholar 6 Custovic A, Fletcher A, Pickering CAC, Francis HC, Green R, Smith A, Chapman M, Woodcock A. Domestic allergens in public places III: House dust mite, cat, dog and cockroach allergens in British hospitals. Clin Exp Allergy 1998; 28: 53–59. 10.1046/j.1365-2222.1998.00183.x CASPubMedWeb of Science®Google Scholar 7 Fincher EL. Aerobiology and hospital sepsis. In RL Dimmick, AB Akers, editors. An introduction to experimental aerobiology. New York: Wiley Interscience; 1969. Google Scholar 8 Zacharisen MC, Kadambi AR, Schlueter DP, Kurup VP, Shack JB, Fox JL, Anderson HA, Fink JN. The spectrum of respiratory disease associated with exposure to metal working fluids. J Occup Environ Med 1998; 40: 640–647. 10.1097/00043764-199807000-00010 CASPubMedWeb of Science®Google Scholar 9 Brachman PS, Kaufmann AF, Dalldorf FG. Industrial inhalation anthrax. Bacteriol Rev 1966; 30: 646–659. 10.1128/MMBR.30.3.646-659.1966 CASPubMedWeb of Science®Google Scholar 10 Kullman GJ, Thorne PS, Waldron PF, Marx JJ, Ault B, Lewis DM, Siegel PD, Olenchock SA, Merchant JA. Organic dust exposures from work in dairy barns. Am Ind Hyg Assoc J 1998; 59: 403–413. 10.1080/15428119891010668 CASPubMedWeb of Science®Google Scholar 11 Wathes CM. Bioaerosols in animal houses. In: CS Cox, CM Wathes, editors. Bioaerosols handbook. Boca Raton: Lewis; 1995. chap. 20. Google Scholar 12 Pillai SD, Widmer KW, Dowd SE, Ricke SC. Occurrence of airborne bacteria and pathogen indicators during land application of sewage sludge. Appl Env Microbiol 1996; 62: 296–299. CASPubMedWeb of Science®Google Scholar 13 Marchand G, Lavoie J, Lazure L. Evaluation of bioaerosols in a municipal solid waste recycling and composting plant. J Air and Waste Manage Assoc 1996; 45: 778–781. 10.1080/10473289.1995.10467406 Web of Science®Google Scholar 14 Rahkonen P, Ettala M, Laukkanen M, Salkinoja-Salonen M. Airborne microbes and endotoxins in the work environment of two sanitary landfills in Finland. Aerosol Sci Technol 1990; 13: 505–513. 10.1080/02786829008959465 Web of Science®Google Scholar 15 Laitinen S, Nevalainen A, Kotimaa M, Liesivuori J, Martikainen P. Relationship between bacterial counts and endotoxin concentrations in the air of wastewater treatment plants. Appl Environ Microbiol 1992; 58: 3774–3776. CASPubMedWeb of Science®Google Scholar 16 Stockholm International Peace Research Institute. The problem of chemical and biological weapons. Vol. 1. The rise of CB weapons. Stockholm: Almqvist and Wiksell; 1971. p 111–124. Google Scholar 17 Messelson M, Guillemin J, Hugh-Jones M, Langmuir A, Popova I, Shelokov A, Yampolskaya O. The Sverdlovsk anthrax outbreak of 1979. Science 1994; 266: 1202–1208. 10.1126/science.7973702 PubMedWeb of Science®Google Scholar 18 Henderson DA. The looming threat of bioterrorism. Science 1999; 283: 1279–1282. 10.1126/science.283.5406.1279 CASPubMedWeb of Science®Google Scholar 19 U.S. Congress, Office of Technology Assessment. Technologies underlying weapons of mass destruction (OTA-BP-ISC-115). Washington, DC: U. S. Government Printing Office; 1993. p 71–117. Google Scholar 20 Hewish M. On alert against the bio agents: Tactical biological-agent detection approaches reality. Jane's Int Def Rev 1998; 31: 53–57. Google Scholar 21 Nevalainen A, Pastuszka J, Liebhaber F, Willeke K. Performance of bioaerosol samplers: Collection characteristics and sampler design considerations. Atmos Environ 1992; 26A: 531–540. 10.1016/0960-1686(92)90166-I CASWeb of Science®Google Scholar 22 Buttner MP, Stetzenbach LD. Monitoring airborne fungal spores in an experimental indoor environment to evaluate sampling methods and the effects of human activity on air sampling. Appl Environ Microbiol 1993; 59: 219–226. 10.1128/AEM.59.1.219-226.1993 CASPubMedWeb of Science®Google Scholar 23 Alvarez AJ, Buttner MP, Toranzos GA, Dvorsky EA, Toro A, Heikes TB, Mertikas-Pifer LE, Stetzenbach LD. Use of solid-phase PCR for enhanced detection of airborne microorganisms. Appl Environ Microbiol 1994; 60: 374–376. 10.1128/AEM.60.1.374-376.1994 CASPubMedWeb of Science®Google Scholar 24 Stewart SL, Grinshpun SA, Willeke K, Terzieva S, Ulevicius V, Donnelly J. Effect of impact stress on microbial recovery on an agar surface. Appl Environ Microbiol 1995; 61: 1232–1239. 10.1128/aem.61.4.1232-1239.1995 CASPubMedWeb of Science®Google Scholar 25 Terzieva S, Donnelly J, Ulevicius V, Grinshpun SA, Willeke K, Stelma GN, Brenner KP. Comparison of methods for detection and enumeration of airborne microorganisms collected by liquid impingement. Appl Environ Microbiol 1996; 62: 2264–2272. 10.1128/AEM.62.7.2264-2272.1996 CASPubMedWeb of Science®Google Scholar 26 Reponen TA, Gazenko SV, Grinshpun SA, Willeke K, Cole EC. Characteristics of airborne actinomycete spores. Appl Environ Microbiol 1998; 64: 3807–3812. CASPubMedWeb of Science®Google Scholar 27 Pinnick RG, Hill SC, Nachman P, Pendleton JD, Fernandez GL, Mayo MW, Bruno JG. Fluorescence particle counter for detecting airborne bacteria and other biological particles. Aerosol Sci Technol 1995; 23: 653–664. 10.1080/02786829508965345 CASWeb of Science®Google Scholar 28 Hairston PP, Ho J, Quant FR. Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence. J Aerosol Sci 1997; 28: 471–482. 10.1016/S0021-8502(96)00448-X CASPubMedWeb of Science®Google Scholar 29 Seaver M, Eversole JD, Hardgrove JJ, Cary WK, Roselle DC. Size and fluorescence measurements for field detection of biological aerosols. Aerosol Sci Tech 1999; 30: 174–185. 10.1080/027868299304769 CASWeb of Science®Google Scholar 30 Jeys T. MIT Lincoln Lab., Technical Report ESC-TR-97-136; 1998. Google Scholar 31 Hill SC, Pinnick RG, Nachman P, Chen G, Chang RK, Mayo MW, Fernandez GL. Aerosol-fluorescence spectrum analyzer: Real-time measurement of emission spectra of airborne biological particles. Appl Opt 1995; 34: 7149–7155. 10.1364/AO.34.007149 CASPubMedWeb of Science®Google Scholar 32 Nachman P, Chen G, Pinnick RG, Hill SC, Chang RK, Mayo MW, Fernandez GL. Conditional-sampling spectrograph detection system for fluorescence measurements of individual airborne biological particles. Appl Opt 1996; 35: 1069–1076. 10.1364/AO.35.001069 CASPubMedWeb of Science®Google Scholar 33 Chen G, Nachman P, Pinnick RG, Hill SC, Chang RK. Conditional-firing aerosol-fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm ultraviolet laser excitation. Opt Lett 1996; 21: 1307–1308. 10.1364/OL.21.001307 CASPubMedWeb of Science®Google Scholar 34 Pinnick RG, Hill SC, Nachman P, Videen G, Chen G, Chang RK. Aerosol fluorescence spectrum analyzer for rapid measurement of single micrometer-sized airborne biological particles. Aerosol Sci. Technol 1998; 28: 95–104. 10.1080/02786829808965514 CASWeb of Science®Google Scholar 35 Pan YL, Holler S, Chang RK, Hill SC, Pinnick RG, Niles S, Bottiger JR. Single shot fluorescence spectra of individual micro-sized bio-aerosols illuminated by a 351 nm or 266 nm laser. Opt Lett 1999; 24: 116–119. 10.1364/OL.24.000116 CASPubMedWeb of Science®Google Scholar 36 Pan YL, Hill SC, Pinnick RG, Niles S, Holler S, Chang RK, Bottiger JR, Roselle DC, Eversole JD, Seaver M. Fluorescence spectra of individual flowing airborne biological particles measured in real time. Am Ind Hyg Assoc J; submitted. Google Scholar 37 Cheng YS, Barr EB, Fan BJ, Hargis PJ, Rader DJ, O'Hern TJ, Torczynski JR, Tisone GC, Preppernau BL, Young SA, Radloff RJ. Detection of bioaerosols using multiwavelength UV fluorescence spectroscopy. Aerosol Sci Tech 1999; 30: 186–201. 10.1080/027868299304778 CASWeb of Science®Google Scholar 38 Gieray RL, Reilly PTA, Yang M, Whitten WB, Ramsey JM. Real-time detection of individual airborne bacteria. J Microbiol Meth 1997; 29: 191–199. 10.1016/S0167-7012(97)00049-3 Web of Science®Google Scholar 39 Kishnamurthy T, Ross PL, Rajamani U. Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 1996; 10: 883–888. 10.1002/(SICI)1097-0231(19960610)10:8 3.0.CO;2-V PubMedWeb of Science®Google Scholar 40 Arnold RJ, Reilly JP. Fingerprint matching of E-coli strains with matrix-assisted laser desorption ionization time-of-flight mass spectrometry of whole cells using a modified correlation approach. Rapid Commun Mass Spectrom 1998; 12: 630–636. 10.1002/(SICI)1097-0231(19980529)12:10 3.0.CO;2-0 CASPubMedWeb of Science®Google Scholar 41 Easterling ML, Colangelo CM, Scott RA, Amster IJ. Monitoring protein expression in whole bacterial cells with MALDI time-of-flight mass spectrometry. Anal Chem 1998; 70: 2704–2709. 10.1021/ac971344j CASPubMedWeb of Science®Google Scholar 42 Dai YQ, Li L, Roser DC, Long SR. Detection and identification of low-mass peptides and proteins from solvent suspensions of Escherichia coli by high performance liquid chromatography fractionation and matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom 1999; 13: 73–78. 10.1002/(SICI)1097-0231(19990115)13:1 3.0.CO;2-N CASPubMedWeb of Science®Google Scholar 43 Snyder AP, Smith PBW, Dworzanski JP, Meuzelaar HLC. Pyrolysis-gas chromatography-mass spectrometry-detection of biological warfare agents. Mass Spectrom Character Microorg 1994; 541: 62–84. 10.1021/bk-1994-0541.ch005 CASWeb of Science®Google Scholar 44 Beverly MB, Basile F, Voorhees KJ, Hadfield TL. A rapid approach for the detection of dipicolinic acid in bacterial spores using pyrolysis mass spectrometry. Rapid Commun Mass Spectrom 1996; 10: 455–458. 10.1002/(SICI)1097-0231(19960315)10:4 3.0.CO;2-Y CASPubMedWeb of Science®Google Scholar 45 Snyder AP, Thornton SN, Dworzanski JP, Meuzelaar HLC. Detection of the picolinic acid biomarker in Bacillus spores using a potentially field-portable pyrolysis gas chromatography ion mobility spectrometry system. Field Anal Chem Technol 1996; 1: 49–59. 10.1002/(SICI)1520-6521(1996)1:1 3.0.CO;2-9 CASWeb of Science®Google Scholar 46 Dworzanski JP, McClennen WH, Cole PA, Thornton SN, Meuzelaar HLC, Arnold NS, Snyder AP. Field-portable automated pyrolysis-GC/IMS system for rapid biomarker detection in aerosols: A feasibility study. Field Anal Chem Technol 1997; 1: 295–305. 10.1002/(SICI)1520-6521(1997)1:5 3.0.CO;2-T CASWeb of Science®Google Scholar 47 Murrell WG. Chemical composition of spores and spore structures. In: GW Gould, A Hurst, editors. The Bacterial Spore. New York: Academic; 1969. p 218–231 and Table III. Google Scholar 48 Setlow P. Germination and outgrowth. In: A Hurst, GW Gould, editors. The bacterial spore. New York: Academic; 1971. vol 2, p 214–217. Google Scholar 49 Chance B. Spectrophotometric and kinetic studies of flavoproteins in tissues, cell suspensions, mitochondria and their fragments. In EC Slater, editors. Flavins and flavoproteins. New York: Elsevier; 1996. p 496–510. Google Scholar 50 Lakowicz JR. Principles of fluorescence spectroscopy. New York: Plenum; 1983. p 341. 10.1007/978-1-4615-7658-7_11 Google Scholar 51 Aubin JE. Autofluorescence of viable cultured mammalian cells. J Histochem Cytochem 1979; 27: 36–43. 10.1177/27.1.220325 CASPubMedWeb of Science®Google Scholar 52 Benson RC, Meyer RA, Zaruba ME, McKhann GM. Cellular autofluorescence: Is it due to flavins? J Histochem Cytochem 1979; 27: 44–48. 10.1177/27.1.438504 CASPubMedWeb of Science®Google Scholar 53 Seaver M, Roselle DC, Pinto JF, Eversole JD. Absolute emission spectra from Bacillus subtilis and Escherichia coli vegetative cells in solution. Appl Opt 1998; 37: 5344–5347. 10.1364/AO.37.005344 CASPubMedWeb of Science®Google Scholar 54 A two-lens system with f/4 collection was used. Google Scholar 55 We used a flash-lamp-pumped, frequency-quadrupled Nd:YAG laser having a non-Gaussian spatial profile, and significant shot-to-shot variations in intensity. Google Scholar 56 Bottiger JR, Deluca PJ, Stuebing EW, VanReenen DR. An ink jet aerosol generator. J Aerosol Sci 1998; 29(suppl 1), s965–966. 10.1016/S0021-8502(98)90665-6 CASGoogle Scholar 57 The Q-switched UV laser is either the 266-nm, fourth harmonic of a Nd:YAG laser [30- or 70-ns pulse duration (Spectra Physics models X-30 or Y-70)], or the 351-nm, third-harmonic of a Nd:YLF laser [120-ns pulse duration (Quantronix)]. The Q-switched laser fires within about 3 μs of the trigger pulse, during which time the particle travels (about 10 m/s) less than 40 μm. This vertical displacement is compensated for by a small vertical displacement of the focal volume of the two diode-laser beams from the UV laser beam (which is focused to the focal point of the reflecting objective). Google Scholar 58 The ICCD camera (Princeton Instruments) is placed at the exit port of the spectrograph (Acton model SP-150 with 300 groove/mm grating blazed at 500 nm, numerical aperture 0.125, input slit width 1 mm). The ICCD detector's image intensifier acts as a fast shutter, opening when the targeted particle is illuminated by the UV laser. A long-pass filter is placed in front of the spectrograph to block elastically scattered light and to pass the fluorescence. Google Scholar 59 To estimate these fractions, the colony-forming units of bacteria and fungi were measured, and the masses were estimated, assuming spherical 1-μm-diameter bacteria at densities of 1 g/cm3. Google Scholar 60 Sidestream cigarette smoke has been measured61 to have a log-normal size distribution with number concentration ≈3×1010 cm−3, geometric mean diameter ≈0.18 μm, and log-normal standard deviation ≈0.4. Thus we would expect the ≈100 particles to occupy a spherical sample volume with 10-μm radius. Google Scholar 61 Okada T, Matsunuma K. Determination of particle-size distribution and concentration of cigarette smoke by a light-scattering method. J Colloid Interface Sci 1974; 48: 461–469. 10.1016/0021-9797(74)90190-8 Web of Science®Google Scholar 62 Faris GW, Copeland RA, Mortelmens K, Bronk BV. Spectrally resolved absolute fluorescence cross sections for bacillus spores. Appl Opt 1997; 36: 958–967. 10.1364/AO.36.000958 CASPubMedWeb of Science®Google Scholar 63 Gelbachs and Birnbaum (Ref. 64) used the 488-nm line of an argon-ion laser to excite fluorescence from atmospheric aerosols. They spectrally dispersed the fluorescence into four large bands, but were unable to obtain detailed spectra or single particle spectra. They noted that if "one type of aerosol predominates, such as cigarette smoke or pollen, a low resolution spectrum," may be adequate. They also suggested that such an instrument may be useful for detecting and identifying aerosol emissions (e.g., fly ash (Ref. 65)) from stacks. Google Scholar 64 Gelbwachs J, and Birnbaum M. Fluorescence of a atmospheric aerosols and lidar implications. Appl Opt 1973; 12: 2442–2447. 10.1364/AO.12.002442 CASPubMedWeb of Science®Google Scholar 65 Birnbaum M. Laser-excited fluorescence techniques in air pollution monitoring. In: EL Wehry, editor. Modern Fluorescence Spectroscopy, Vol. 1. New York: Plenum; 1976. Chapter 5. Web of Science®Google Scholar 66 Nakamura S, Fasol G. The blue green diode. Berlin: Springer; 1997. 10.1007/978-3-662-03462-0 Google Scholar Citing Literature Volume3, Issue4-5Special Issue: Chemical and Biological Aerosol Detection and Identification with Field Analytical Instrumentation1999Pages 221-239 ReferencesRelatedInformation