Produção Nacional
Revisado por pares
Critical involvement of Th1-related cytokines in renal injuries induced by ischemia and reperfusion
2008; Elsevier BV; Volume: 9; Issue: 6 Linguagem: Inglês
10.1016/j.intimp.2008.11.012
ISSN1878-1705
AutoresVanessa Nunes de Paiva, Rebecca Midory Marques Monteiro, Vilmar de Paiva Marques, Marcos Antônio Cenedeze, Vicente de Paula Antunes Teixeira, Marlene Antônia dos Reis, Álvaro Pacheco‐Silva, Niels Olsen Saraiva Câmara,
Tópico(s)Thermal Regulation in Medicine
ResumoRenal ischemia and reperfusion injury (IRI) is considered an inflammatory syndrome. To move forward in its pathogenesis, we exploited the role of several cytokines on renal damages triggered by IRI. Specifically to evaluate the role of Th1 immune profile in this system, IL-12, IFN-γ, and IFN-γ/IL-12 deficient (KO) mice on C57BL/6 background and their controls were subjected to IRI. In each group, blood and kidney samples were harvested. Renal function was evaluated by serum creatinine and renal morphometric analyses. Gene expression of IL-6 and HO-1 were also investigated by Q-PCR. IFN-γ KO animals presented the highest impairment in renal function compared to controls. Conversely, IL-12 KO animals were absolutely protected and, in a lesser extent, IFN-γ/IL-12 KO double knockout was also protected from IRI. Gene expression analyses showed higher expression of HO-1, a cytoprotective gene, and IL-6, a pro-inflammatory cytokine, in IFN-γ deficient animals subjected to IRI. Our results confirm that Th1 related cytokines such as IL-12 and IFN-γ are critically involved in renal ischemia and reperfusion injury.
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