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Studies of DNA-strand breaks induced in human fibroblasts by chemical mutagens/carcinogens

1979; Elsevier BV; Volume: 63; Issue: 2 Linguagem: Inglês

10.1016/0027-5107(79)90071-x

1873-135X

Magnus Nordenskjöld, S Söderhäll, Peter Moldéus,

Plant Genetic and Mutation Studies

A method for the study of DNA-strand breaks using alkaline denaturation followed by hydroxylapatite chromatography has been modified and used for the detection of chemically induced DNA-strand breaks. A new procedure for the incubation of human fibroblasts with a metabolizing system and the detection of DNA-strans breaks is presented. With this method the induction and repair of DNA-strand breaks have been studied in human fibroblasts exposed to methyl methanesulphonate, melphalan, benzo[a]pyrene and cyclophosphamide. These agents all give rise to DNA-strand breaks. In cells exposed to methyl methanesulphonate, melphalan or benzo[a]pyrene these breaks disappeared within 21 h after re moval of the drug. In cells exposed to the bifunctional alkylating agent cyclophosphamide, studies of DNA-strand breaks suggest the presence of inter-strand cross links.