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Meiotic irregularities and pollen viability in Passiflora edmundoi Sacco (Passifloraceae)

2003; University of Florence; Volume: 56; Issue: 2 Linguagem: Inglês

10.1080/00087114.2003.1010589320

2165-5391

Margarete Magalhães Souza, Telma Nair Santana Pereira, Alexandre Pio Viana, Messias Gonzaga Pereira, Luís Carlos Bernacci, Cláudia Pombo Sudré, Leandro da Cruz Silva,

Chromosomal and Genetic Variations

Abstract Meiotic and pollen viability studies were conducted in Passiflora edmundoi Sacco (n = x = 9). For meiotic studies anthers were squashed in a drop of 1% acetic carmine. For analysis of pollen viability, pollen grains of flowers at anthesis were stained with two stains, lugol and Alexander stain. At diakinesis frequency of bivalents was high (86% of pollen mother cell observed) and it was observed other pairing patterns (7 II + 1 IV; 5 II + 2 IV; and 18 I). The average number of chiasmata per cell was 9.3, in concordance with high frequency of bivalents. Meiotic phases showed the presence of spindle irregularities, asynchrony, and other abnormalities as laggard chromosomes and anaphase bridges. Different post‐meiotic products were observed and the frequency of triads was higher than monads, dyads, and polyads; however the meiotic index was 97.2% and the pollen viability was 97.9% suggesting meiotic stability. Sterile pollen was sorted in two categories: empty (60.3%) and shrunken (contracted cytoplasm) grains. The high meiotic index, pollen viability, frequency of chiasmata and bivalent pairing indicate that the species is meiotic stable and can be used in selection programs and intra and inter‐specific crosses beyond the meiotic irregularities observed.