Produção Nacional
Callus induction and plant regeneration by Brazilian triticale and wheat genotypes
1997; Brazilian Society of Genetics; Volume: 20; Issue: 1 Linguagem: Inglês
10.1590/s0100-84551997000100008
ISSN1678-4502
AutoresAna Lúcia Cunha Dornelles, Fernando Irajá Félix de Carvalho, Luiz Carlos Federizzi, Cristine Luise Handel, Fernanda Bered, Maria Elena Basilio Sordi, Fernanda Schneider,
Tópico(s)Turfgrass Adaptation and Management
ResumoIn order to determine the in vitro behavior of Brazilian triticale, 16 triticale genotypes, and three wheat genotypes used as checks, were sown in June 1994. The explants used were immature embryos. In addition to the genotype tests, two culture media for callus induction were also evaluated, i.e., MS (Murashige and Skoog, Physiol. Plant. 15: 473-497, 1962) medium containing 2.0 mg 2,4D/l, and MS medium containing 4.0 mg 2,4D/l. The plant regeneration protocol used was the one employed at the Laboratório de Cultura de Tecidos, Departamento de Plantas de Lavoura, Universidade Federal do Rio Grande do Sul, for wheat. Differences in plant regeneration were observed both among triticale and wheat genotypes, with triticale usually showing better regeneration than wheat. No differences were observed between the callus induction media.
Referência(s)