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Association study of CCR5 delta 32 polymorphism among the HLA‐DRB1 Caucasian population in Northern Paraná, Brazil

2008; Wiley; Volume: 22; Issue: 4 Linguagem: Inglês

10.1002/jcla.20225

1098-2825

Sandra Márcia Muxel, Sueli Donizete Borelli, Marla Karine Amarante, Júlio César Voltarelli, Mateus Nóbrega Aoki, Carlos Eduardo Coral de Oliveira, Maria Angélica Ehara Watanabe,

Immune Cell Function and Interaction

Abstract Chemokines are important determinants of early inflammatory response. The CC chemokine receptor 5 (CCR5) delta 32 variant results in a nonfunctional form of the chemokine receptor and has been implicated in a variety of immune‐mediated diseases. In the present study, polymerase chain reaction (PCR) for genomic deoxyribonucleic acid (DNA) samples, using specific CCR5 oligonucleotide primers surrounding the breakpoint deletion, detected a 225‐basepair (bp) product from the normal CCR5 allele and a 193‐bp product from the 32 bp deletion allele. Human leukocyte antigen (HLA) class II (DRB1) typing was performed by PCR‐sequence‐specific primer (PCR‐SSP). The aim of this study was to evaluate the association of HLA‐DRB1 and CCR5 genetic polymorphisms. To evaluate the frequency distributions of CCR5 delta 32 polymorphisms in a Brazilian population and their association with allelic distribution of HLA genes, DRB1; a total of 120 Caucasian individuals from northern Paraná, Brazil, were tested. The CCR5/CCR5 genotype was found in 108 individuals (90%) and only one carried the CCR5 delta 32 allele homozygous genotype (0.0238), while 12 (10%) carried the CCR5 delta 32 allele heterozygous genotype. The observed frequency for the CCR5 delta 32 allele was 0.05 in the population studied. The results revealed a CCR5 delta 32 allele occurrence with HLA‐DRB1 * 01 and DRB1 * 04 ( P <0.05). It is possible that HLA‐DRB1 * 01 and DRB1 * 04 alleles could be associated with the delta 32‐bp deletion of CCR5. J. Clin. Lab. Anal. 22:229–233, 2008. © 2008 Wiley‐Liss, Inc.