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Effects of various barriers on platelet-activating factor-induced neutrophil chemotaxis

1991; Elsevier BV; Volume: 87; Issue: 2 Linguagem: Inglês

10.1016/0091-6749(91)90016-h

1097-6825

Thomas B. Casale, Mohammed Abbas,

Protease and Inhibitor Mechanisms

Platelet-activating factor (PAF)-induced neutrophil migration across endothelial and epithelial barriers is an important event in inflammation.We compared the effects of PAF on human neutrophil migration through jilters alone, and human umbilical vein endothelial and Madin-Darby canine kidney epithelial cells cultured on these filters.We found that the amount of PAF-induced neutrophil migration was barrier dependent.At low PAF concentrations, neutrophil migration through filters exceeded migration measured through either cellular barrier.As PAF concentrations increased to ~1 pmollL, neutrophil migration through human umbilical vein endothelial cells often equaled or exceeded migration measured through Jilters alone.In contrast, sign&ant neutrophil migration through Madin-Darby canine kidney cells was not observed until PAF concentrations exceeded 50 prnollL.Thus, the intensity of PAP-induced neutrophil inflammatory processes is, in part, dependent on the barrier through which the neutrophils must migrate.(J ALLERGY CLIN IUMUNOL 1991;87:565-74.)Neutrophils are the predominant phagocytic cells of the blood and are usually the first cells to reach the sites of infection or inflammation.The emmigration of neutrophils from blood into tissues involves the chemotactically mediated transmigration of neutrophils across endothelial and epithelial barriers.The ability of chemoattractants to induce neutrophil migration depends on the nature of the chemoattractant and the interaction of the chemoattractant with specific cell-surface receptors.1 Many inflammatory mediators, including LTB, and PAF, have been demonstrated to induce neutrophil migration across various cellular and noncellular barriers.le6However, little is known about the effects of the barrier on mediator-induced NCR.To address this issue, we directly compared the ability of PAF to induce neutrophil migration across filters alone and monolayers of endothelial and epithelial cells cultured on these filters.