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Microscopics of Complexation between Long DNA Molecules and Positively Charged Colloids

2002; American Physical Society; Volume: 89; Issue: 8 Linguagem: Inglês

10.1103/physrevlett.89.088103

1092-0145

Kinneret Keren, Yoav Soen, G. Ben Yoseph, Rachel Gilad, Erez Braun, Uri Sivan, Yeshayahu Talmon,

Electrostatics and Colloid Interactions

Extensive atomic force and electron microscopy reveal a new, generic DNA-colloid complex with a fixed number of DNA bases per colloid. The fiber shaped complex is stable in the presence of excess colloids in the solution. As more DNA is added to the solution and the ratio between colloids and DNA approaches the fiber's stoichiometry, the system undergoes a sharp coagulation transition. The system is restabilized at even higher DNA concentrations through localization of small colloid clusters on extensive DNA networks.