Identification of neuropeptide FF‐related peptides in human cerebrospinal fluid by mass spectrometry
2002; Wiley; Volume: 532; Issue: 3 Linguagem: Inglês
10.1016/s0014-5793(02)03686-4
ISSN1873-3468
AutoresOdile Burlet‐Schiltz, Honoré Mazarguil, Jean‐Christophe Sol, Patrick Chaynes, Bernard Monsarrat, Jean‐Marie Zajac, Anne Roussin,
Tópico(s)Hypothalamic control of reproductive hormones
ResumoSeveral neuropeptide FF (NPFF)‐related peptides, known as modulators of the opioid system, have been previously characterized in bovine and rodent brain. Reverse‐phase high pressure liquid chromatography (HPLC) fractions of a human with normal pressure hydrocephalus cerebrospinal fluid (CSF), co‐migrating with NPFF‐related synthetic peptides, were characterized by capillary HPLC coupled on‐line to nanospray ion trap tandem mass spectrometry. Two peptides present in the pro‐NPFF A precursor, NPAF (AGEGLNSQFWSLAAPQRF‐NH 2 ) and NPSF (SLAAPQRF‐NH 2 ), were identified. The monitoring of NPFF‐related peptides in human CSF can be helpful to understand their roles in pain sensitivity.
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